金鼎生物科技有限公司


Kinetic Biotechnology Co., Ltd.
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PCR Master Mix

FIREPol® 5 x Master Mix Ready to Load

7.5 / 12.5  mM MgCl2

 

For in vitro use only

Description:
 

5x FIREPol® Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). Ready to Load format also includes a compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.


 

We recommend using 5x FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. 5x FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.

Applications:

- Suited for a wide range of PCR assays
 

- Primer extension

 

Reagent Composition:

- FIREPol® DNA polymerase

- 5x Reaction Buffer B 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20 

- 7.5 / 12.5  mM MgCl2 1x PCR solution – 1.5 / 2.5 mM MgCl2 

- 2 mM dNTPs of each  1x PCR solution – 200 µM dATP, 200 µM dCTP,   200 µM dGTP and 200 µM dTTP

- Blue dye Migration equivalent to 3.5-4.5 kb DNA fragment

- Yellow dye  Migration rate in excess of primers in 1% agarose gel: <35-45 bp

- Compound that increases sample density for direct loading


 

Advantages of Ready-to-Load Mix

- decreased set-up time
 

- reduced risk of contamination
 

- less pipetting mistakes
 

- consistent reaction to reaction performance
 

- contains all reagents required for PCR

Like all our products, the 5x FIREPol® Master Mix Ready to Load is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.


 

Shipping and Storage Conditions:

Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of FIREPol® 5x Master Mix Ready to Load.

 

Ordering Information:

Cat. No. Product Packing Size Data Sheet MSDS Price(NT)
04-12-00115 5x FIREPol® Master Mix Ready to Load (7.5 mM MgCl2)

250 reactions (1 ml)

1,200
04-12-00125 5x FIREPol® Master Mix Ready to Load (12.5 mM MgCl2)

250 reactions (1 ml)

1,200