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Kinetic Biotechnology Co., Ltd.
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DNA Polymerase

 

FIREPol® DNA Polymerase

 

 For in vitro use only

 

Description:

FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol® has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

Applications:

- Suited for a wide range of PCR assays
 

- Primer extension
 

- TA cloning


 

Concentration:

5 U/µl


 

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.

 

Reagents Provided:

- FIREPol® DNA Polymerase

- 10x Reaction buffer B (Mg2+ free) 

0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20

- 10x Reaction buffer BD (Mg2+ and detergent free)

0.8 M Tris-HCl, 0.2 M (NH4)2SO4

25 mM MgCl2

- 10x Solution S Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution).
Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.


 

Shipping and Storage Conditions:

Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® DNA Polymerase.

 

Ordering Information:

Cat. No. Product Packing Size Data Sheet MSDS Price(NT)
01-01-00500

FIREPol® DNA Polymerase(5 U/μl)

500 U

1,000