金鼎生物科技有限公司


Kinetic Biotechnology Co., Ltd.
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PCR Reagent

  • DNA Polymerase

    FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol® has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity. 

  • Hot Start DNA Polymerase

    Hot Start PCR prevents misextension from non-specifically annealed primers and primer-dimers. HOT FIREPol®  DNA Polymerase is a chemically modified FIREPol®  DNA Polymerase. At ambient temperature it is inactive having no polymerization activity. HOT FIREPol® DNA Polymerase is activated by a 12-15 min incubation step at 95º C 

  • PCR Master Mix

    5x FIREPol® Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). We recommend using 5x FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining.

  • Pfu and Hot Start Master Mix

    5x HOT FIREPol® Blend Master Mix is a highly user-friendly and versatile tool for your Hot Start PCR. The mix is based on a blend of our HOT FIREPol® polymerase and a proofreading polymerase, providing a low error rate and high sensitivity. In addition, the mix also allows amplification of longer fragments (up to 5 kb). Here some great advantages of this ready-to-load mix: 

    • decreased set-up time
    • reduced risk of contamination
    • less pipetting mistakes
    • consistent reaction to reaction performance
    • contains all reagents required for PCR

    Like all our products, the 5x HOT FIREPol® Blend Master Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.